Applied Electrospray Mass Spectrometry: Practical by Birendra N. Pramanik, A.K. Ganguly, Michael L. Gross

By Birendra N. Pramanik, A.K. Ganguly, Michael L. Gross

Discussing ideas to figure out the constitution and machanisms of diverse compound classics, this publication covers new chemical and elctrophoretic options for speedy pattern preconcentration and separation. It summarizes breakthroughs within the concept and instrumentation of electrospray mass spectrometry in pharmaceutical and biomedical functions, presents functional examples for the characterization of peptides, proteins, and glycoproteins, contains purposes in proteomics, combinatorial chemistry, and drug characterization. issues comprise chemical and electrophoretic concepts for quick pattern preconcentration and separation, screening methods for proteins from libraries of compounds, protein folding and dynamics, and extra

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Extra resources for Applied Electrospray Mass Spectrometry: Practical Spectroscopy Series Volume 32

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The detection limits for electron ionization mass spectrometry or electron Copyright © 2002 by Taylor & Francis capture negative ion mass spectrometry can be in the low femtogram range. In a recent study, we evaluated both GC/MS and LC/MS methods for analyzing compounds suspected to have endocrine-disrupting activity (338). Both approaches were successful and gave comparable detection limits. A benefit of the LC/MS approach is that sample derivatization is not required. III. MASS ANALYZERS The operating principles of the various mass analyzers have been discussed at length in numerous other books and reviews, and a detailed discussion of each analyzer is beyond the scope of this chapter.

242) have also applied ultrasonic nebulization to ESI-MS. This combination, termed Ultraspray, was trademarked and commercialized by Analytica of Branford and showed some performance advantages over pneumatic nebulization (243). Copyright © 2002 by Taylor & Francis However, the added cost and complexity of the ultrasonic spray needle and power supply, together with the difficulty in replacing the needle in the event of clogging, have limited the popularity of this approach. d. ). Numerous variations on the ESI source design have been developed in an attempt to reduce ion source contamination and improve the ability of ESI to work with nonvolatile buffers.

The maximum achievable collision energy in FTICR decreases with increasing massto-charge ratio and increases with increasing magnetic field. High energy CID is possible for small m/z ions, but it is not generally practical for a full mass range. McLafferty and coworkers (408) reported an intriguing alternative to CID that is based upon electron capture by large, multiply charged ions. Multistage mass spectrometry experiments in FTICR and quadrupole ion trap mass spectrometers are referred to as “tandem-in-time” as opposed to the “tandem-in-space” experiments performed with magnetic sector and quadrupole mass spectrometers.

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