Analytical Applications of Circular Dichroism by N. Purdie, Harry G. Brittain

By N. Purdie, Harry G. Brittain

Good points specific points of round dichroism and examines the way it might be of tips to employees within the box. The textual content discusses the actual phenomena linked to dissymmetric compounds which offer upward thrust to the round dichroism impression, and studies the instrumentation on hand.

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131. comparison of Fig. 1, which depicts a conventional CD system, and Fig. 4 shows the primary difference in the two approaches to be in the type and location of the wavelength dispersion device. For multichannel detection, the monochromator has been replaced by a polychromator which does not possess an exit slit to define a wavelength interval. For multichannel detection, the location (pixel) on the multichannel device defines the wavelength of interest. In conventional approaches, the monochromator is placed after the source in order to limit the UV exposure of the sample.

Fig. 2 schematicallyrepresents Cotton's instrumental system for measuring CD, which separately determines the sample absorption coefficients for left- and right-circularly polarized light. In Fig. 2, light from a polychromatic source is split between two distinct paths; one of which includes the sample and one which passes solely through a pair of Nicol polarizing prisms. The combination of the first Nicol prism (linear polarizer) and the Fresnel rhomb (circular polarizer) converts the source light into one sense of circularly polarized light.

As was pointed out above, the Pockels cell places fairly severe limitations on the spatial characteristics of the light beam to be modulated. In this system, in spite of the fact that a longitudinal arrangement was utilized for the Pockels cell, an acceptance angle of approximately 2 O was required. This necessitated the incorporation of an iris before the Pockels cell to limit the cone of light entering the device. Fig. 4 below schematically depicts the optical arrangement developed by Wetzel and co-workers which was designed to incorporate multichannel detection into the CD experiment.

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