By W. N. Aldridge (eds.)
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Fig. 1 also shows that atropine administered a few minutes before the anti-ChE markedly reduces the hypothermia, although it is unable to prevent it completely. Fig. 1 shows the maximum antidotal effect of atropine obtained following an extremely high dose (50 mgfkg, intraperitoneally). Increasing the dose beyond this level produces no better effect, and may kill the rats. Atropine alone evokes a slight hypothermia. Because the rat has sweat glands only in the soles of the feet, sweating is E. Meeler 32 not an effective means of dissipating excess heat.
IDOl each, were added as markers to the sample. After electrophoresis, 1 em sections of the paper strip were cut out for determinations of radioactivity. The only radioactive components found are those shown in the figure. ) or by heating at I oooc for a few minutes. It is relatively stable at 26°C in strong acid solution. Methionine sulphoximine phosphate is about 50% hydrolysed when heated at 100°C in 1 NHCl for 85 min. Such stability is inconsistent with an a-N-phosphoryl or a carboxyl phosphate derivative, for such compounds are known to be very susceptible to hydrolysis.
Hydrazone formation was measured at 235 nm ( 0) and the ability of the product to inhibit the oxidation of benzylamine by monoamine oxidase is shown (e). change in extinction during the reaction of ,8-phenylethylhydrazine with monoamine oxidase indicate that only negligible quantities of the free aldehyde are produced during the reaction, implying that the rate of hydrazone formation in the presence of the enzyme is greater than the rate of the condensation of aldehyde and hydrazine in its absence.